精细化工

• 生物电催化：酶电极用于医疗诊断食品安全检测、酶电催化高效制备手性化合物
• AI蛋白质设计：人工智能辅助酶设计，极端酶研究，基因组挖掘.
• 体外合成生物学：多酶耦联体系的可控组装与协同催化，无细胞催化体系.
• 生物能源：生物质产氢，体外多酶制氢
• 仿生软材料：抗菌、抗病毒材料、角蛋白等生物材料、仿生矿化等

Immobilization;Kinetics;Biocatalysis;Interaction Network;Biocatalyst;Citalopram;Enzymes;Non-aqueous System;Co-evolution Analysis;Formate Dehydrogenase

1. Yuxin Chen, Qian Zhang, Hangbin Lei, Yulong Han, Shizhen Wang*. Design phenylalanine dehydrogenase for L-homophenylalanine synthesis by conservation and co-evolution analysis. Chemical Engineering Science, 2025,304: 120997
2. Qian Zhang, Yuxin Chen, Lingxuan Duan, Lingling Dong, Shizhen Wang*. Design glutamate dehydrogenase for non-aqueous system by motifs reassembly and interaction network analysis. Journal of Agricultural and Food Chemistry,2024, 72, 19931−19939
3. Hangbin Lei, Qian Zhang, Xiaoyan Xiang, Liang Jiang, Shiyan Wang, Lingxuan Duan, Shizhen Wang*. Metal-organic framework hybrid materials of ZIF-8/RGO for immobilization of D-amino acid dehydrogenase. Nano research 2024,17, 290–296.
4. Lingling Dong, Yu Xiong, Xiaoyan Xiang, Feixuan Li, Qidi Song, Shizhen Wang*. Kinetic and stability studies of amino acid metal-organic frameworks for encapsulating of amino acid dehydrogenase. Journal of Biotechnology, 2024;391,50–56.
5. Zhehui Ji, Qian Zhang, Lingxuan Duan, Lingling Dong, Shizhen Wang*. Design of motifs interfacial interactions by co-evolved analysis of D-amino acid dehydrogenase for stability enhancement. AIChE Journal, 2023; 69(11):e18214.
6. Shizhen Wang*, Hangbin Lei, Zhehui Ji. Exploring oxidoreductases from extremophiles for biosynthesis in a non-aqueous system. International Journal of Molecular Sciences, 2023,24: 6396.
7. Xiaoyan Xiang, Yu Xiong, Qian Zhang, Hangbin Lei, Kailong Liu, Shizhen Wang*. Ionic liquids modified Cu3(PO4)2 hybrid nanoflower for dehydrogenase immobilization by biomimetic mineralization. Process Biochemistry, 2022,121: 584–589.
8. Yu Xiong, Chao Wang, YuanFei Wu, Chunhua Luo*, Dongping Zhan, Shizhen Wang*. Electrochemical enzyme sensor based on the two-dimensional metal–organic layers supported horseradish peroxidase. Molecules, 2022, 27:8599.
9. Zhehui Ji, Heyu Huo, Lingxuan Duan, Shizhen Wang*. Design of robust malate dehydrogenases by assembly of motifs of halophilic and thermophilic enzyme based on interaction network. Biochemical Engineering Journal, 2023,190:108758.
10. Yu Xiong, Liang Jiang, Guang-xiao Yao, Ling-xuan Duan, Shizhen Wang*. Characterization and kinetic study of immobilized of phenylalanine dehydrogenase on metal ions coordinated polydopamine-coated MWNTs. Biochemical Engineering Journal, 2022,180:108370.
11. Zhehui Ji, Guangxiao Yao, Liang Jiang, Wang, Shizhen Wang*. One-pot purification and immobilization of phenylalanine dehydrogenase from Bacillus nanhaiensi by functional reduced graphene oxide. Marine Biotechnology, 2022,24:555–565.
12. Shizhen Wang, Chengwei Ma, AnPing Zeng*. Analysis of co-evolved interaction network of E.coli aspartokinase III and regulation of allosteric progress using energy transduction. Chemical Engineering Journal, 2022, 429:132151.
13. Kailong Liu,Shiyan Wang, Lingxuan Duan, Liang Jiang, Shizhen Wang*. Effect of ionic liquids on catalytic characteristics of hyperthermophilic and halophilic phenylalanine dehydrogenase and mechanism study. Biochemical Engineering Journal, 2021,176:108175
14. Shizhen Wang*, Yu Xiong, Matthew M. Sartin, Dongping Zhan. Research advances in regulating the microenvironment of enzyme electrodes in non-aqueous systems: a mini-review, Electroanalysis, 2022,34: 590
15. Shiyan Wang, Lingxuan Duan, Liang Jiang, Kailong Liu, Shizhen Wang*. Assembly of peptide linker to amino acid dehydrogenase and immobilized with metal–organic framework, Journal of Chemical Technology and Biotechnology, 2021,97: 741-748.
16. Shizhen Wang, Chengwei Ma, AnPing Zeng*. Dynamic energy correlation analysis of E. coli asp-artokinase III and alteration of allosteric regulation by manipulating energy transduction pathways. Engineering in Life Sciences. 2021,21:314-323.
17. Shi-Zhen Wang, Corinne Cruaud, Jean-Marc Aury, David Vallenet, Julie Poulain, Benoit Vacherie, Anne Zaparucha, and Carine Vergne-Vaxelaire*. Complete Genome Sequences of two Pseudomonas species isolated from marine environments of Pacific Ocean. Microbiology Resource Announcement, 2021,10: e01062-19 .
18. Guangxiao Yao, Kai-long Liu, Shiyan Wang, Heyu Huo, Shizhen Wang* Metal ionscoordinated immobilization of phenylalanine dehydrogenase by GO-PEI with high activity recovery and enhanced stability. Journal of Chemical Technology and Biotechnology. 2020, 96:1049-1056.
19. Heyu Huo, Guangxiao Yao, Shizhen Wang* Economy assessment for the chiral amine production with comparison of reductive amination and transamination routes by multi-enzyme system. Catalysts. 2020, 10:1451.
20. 雷航彬,何宁,李斐煊,董玲玲,王世珍*. 氢化酶固定化研究进展. 合成生物学, 2024,5, DOI: 10.12211/2096-8280.2024-022
21. 董玲玲,李斐煊,雷航彬,宋启迪,王世珍*. 仿生分区室固定化多酶体系. 合成生物学, 2024 ,5, DOI: 10.12211/2096-8280.2024-025
22. 段凌暄,姚光晓,江亮,王世珍*.耐有机溶剂氨基酸脱氢酶基因挖掘与非天然氨基酸的非水相合成.化工学报,2021,72(07):3757-3767.
23. 王世珍*,刘凯泷,詹东平.氧化还原酶电催化反应研究进展.科学通报,2021,66(10):1240-1249.
24. 王世珍*,刘凯泷,王世燕,江亮,段凌暄,熊雨,季哲惠.新型材料固定化氧化还原酶研究进展.福州大学学报(自然科学版),2021,49(04):568-576.

1. 国际授权专利Antivirales Breitbandmaterial und Verfahren zu seiner Herstellung und dessen Anwendung(一种广谱抗病毒材料及其制备方法和应用，比利时) BE2021005510,2022-07-20
2. 用于酶电催化还原的氧化还原酶电极及其制备方法和其酶电反应器, 2025-02-11授权，中国，CN202011386220.4
3. 羊毛固定化酶的制备方法及羊毛固定化酶柱反应器，2024-09-10授权，中国，CN202111363662.1.
4. 海洋菌株及其催化制备四氢姜黄素的用途,2024-05-24授权,中国,CN202210413552.X
5. 氧化还原酶及其设计、制备方法与应用. 2024-02-02授权,中国, CN112626042B
6. 一种多肽修饰氨基酸脱氢酶及其制备和固定化方法,2022-11-1, 授权,中国,CN202011034260.2.
7. 一种苯丙氨酸脱氢酶及其制备方法与用途,2022-5-13授权, 中国,CN202010609009.8.
8. 一种胺脱氢酶电极及其制备方法和应用,2020-7-17授权, 中国,CN2019101027540.
9， 一种还原氧化石墨烯固定化苯丙氨酸脱氢酶的方法,2018-12-28, 中国,CN201811621173.
10. 用于酶电催化还原的氧化还原酶电极及其制备方法和其酶电反应器,2020-12-01, 中国,CN202011386220.4.
11. 一种氨基酸脱氢酶及其应用,2021-07-09授权, 中国,201910820939.5.
12. 一种原位分离与固定依赖NADH和NADPH的耐盐氧化还原酶的方法,2021-03-30授权, 中国,CN201910586963.7.
13. 一种胺脱氢酶及其应用,2021-06-08授权, 中国,CN201910820937.6.
14. 一种氨基酸脱氢酶电极及其制备方法和应用,2021-9-13授权, 中国,CN202010542733.3.
15. 一种用于催化制备非天然氨基酸的苯丙氨酸脱氢酶及其应用,2021-03-30授权, 中国,CN201910822218.8.

主持项目：
• 国家基金面上项目“基于电势调控模块组装的超敏别构酶信号-能量耦联传递路径设计” (No.: 22478323).
• 国家重点研发计划合成生物学重点研发项目“糖水氢电系统–体外多酶高效产氢及氢电装置的基础及工程研究”（No.: 2022YFA0912000），子课题负责人.
• 国家基金面上项目“氨基酸脱氢酶分子开关设计与生物电催化不对称还原研究” (No.: 22078273).
• 国家基金面上项目“耐盐氨基酸脱氢酶的抗逆机制研究与元件优化组装” (No.: 21776233).
• 国家青年基金项目“海洋微生物依赖NADH胺脱氢酶的底物特异性研究与催化性能调控” (No.: 41306124).
• 福建省自然基金项目“高苯丙氨酸脱氢酶的底物识别机制研究和催化性能调控” (No.: 2018J01013).
• 中央高校基本科研业务费项目“有机相胺脱氢酶电极可控组装研究及其在食品有机胺检测中的应用” (No. 20720170033).
• 福建省自然基金项目“胺脱氢酶构建多酶耦联新体系催化制备手性氨基醇” (No.:2012J01049).
• 中央高校基本科研业务费项目“多酶耦联生物分子机器制备手性氨基酸” (No.2013121029).

参与项目：
• 国家重点研发计划绿色生物制造重点研发项目“低廉生物质制备全生物基可降解包装材料关键技术”（2022YFC2104600）子课题生物基单体及聚乳酸合成关键技术，参与。
• 国家基金委重点项目“多酶生物分子机器的可控组装与制备药物的生物化工基础” (No.: 21336009).
• 国家自然科学基金面上项目,“地衣芽孢杆菌胞外聚合物合成的调控机制研究” (No.: 21676221)
• 国家基金面上项目“深海细菌Pseudomonas marincola的氧化还原酶高通量挖掘、特异性研究与应用” (No.:41176111).
• 国家海洋局海洋公益性行业科研专项“基于海洋微生物发酵的新产品开发技术研究与应用”子项目苹果酸脱氢酶制备关键工程化技术研究与应用(No.: 201505032-6).
• 国家基金面上项目“并用NADH和NADPH生物转化甘油制备1,3-丙二醇的合成生物系统研究” (No.:21076172).
• 国家基金面上项目“关键基因对微生物胶体稳定性的影响机制与细菌细胞的絮凝行为调控” (No.: 51378444).
• 福建省产学合作重大项目“生物转化生物柴油副产物粗甘油生产1,3-丙二醇的新技术开发” (No.: 2010H6023).
• 厦门市科技计划项目“海洋微藻生产功能保健食品DHA关键技术研发”(No. 201192008146).
• 福建省科技重大专项“微藻提取DHA的技术开发”(No.: 201072001026).

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