精细化工

人工智能/计算机辅助的工业蛋白质科学与生物催化工程：
结合人工智能/计算机辅助技术和生物催化与转化技术进行生物质糖方向酶元件的挖掘、改造、机制解析和应用研究以获得催化机制清晰、限速步骤明确、酶学性质显著提升的关键酶，服务于可持续发展及“碳达峰、碳中和”的国家战略。

Zhiyou Zong, Xinzhuo Liu, Zhuo Ye, Dingbin Liu* A double-switch pHLIP system enables selective enrichment and identification of circulating tumor-derived extracellular vesicles. P Natl Acad Sci, 2023, 120, e2214912120. (IF: 12.779，Q1)
Zhiyou Zong, Scott Mazurkewich, Caroline S. Pereira, Haohao Fu, Wensheng Cai, Xueguang Shao, Munir S. Skaf, Johan Larsbrink, Leila Lo Leggio* Mechanism and biomass association of glucuronoyl esterase: an α/β hydrolase with potential in biomass conversion. Nat Commun, 2022, 13(1): 1-10. (IF: 17.694，Q1)
Zhiyou Zong, Qiyu Li, Zhangyong Hong, Haohao Fu, Wensheng Cai*, Christophe Chipot, Huifeng Jiang, Dongyuan Zhang, Shulin Chen and Xueguang Shao*. Lysine mutation of the claw-arm-like loop accelerates catalysis by cellobiohydrolases. J Am Chem Soc, 2019, 141, 14451-14459. (IF: 16.383，Q1)
Zhiyou Zong, Ronglin He, Haohao Fu, Tanfeng Zhao, Shulin Chen, Xueguang Shao and Wensheng Cai* Pretreating cellulases with hydrophobins for improving bioconversion of cellulose: an experimental and computational study. Green Chem, 2016, 18: 6666-6674. (IF: 11.034, Q1)
Zhiyou Zong, Le Gao, Wensheng Cai, Liang Yu, Chao Cui, Shulin Chen and Dongyuan Zhang* Computer-assisted rational modifications to improve the thermostability of β-glucosidase from Penicillium piceum H16. BioEnerg Res, 2015, 8: 1384-1390. (IF: 3.852, Q2)
Zhiyou Zong, Lijuan Ma, Liang Yu, Dongyuan Zhang, Zhenhua Yang, Shulin Chen* Characterization of the interactions between polyethylene glycol and cellulase during the hydrolysis of lignocellulose. BioEnerg Res, 2015, 8: 270-278. (IF: 3.852, Q2)
Xuehui Liu, Zhiyou Zong, Xinzhuo Liu, Qiang Li, Ang Li, Chen Xu,* and Dingbin Liu* Stimuli-Mediated Specific Isolation of Exosomes from Blood Plasma for High-Throughput Profling of Cancer Biomarkers. Small Methods 2022, 6, 2101234. (IF: 15.367，Q1)
Xinzhuo Liu, Zhiyou Zong, Mindan Xing, Xuehui Liu, Jia Li, Dingbin Liu* pH-Mediated Clustering of Exosomes: Breaking Through the Size Limit of Exosome Analysis in Conventional Flow Cytometry. Nano Lett, 2021, 21, 8817？8823. (IF: 12.262，Q1)
Zhiyou Zong, Qiyu Li, Zhangyong Hong, Wensheng Cai* and Xueguang Shao*. Insight into the exo-exo synergy of Cel7A with Cel6A: an experimental and computational study. Research Square (preprint), https://doi.org/10.21203/rs.3.rs-298025/v1
Le Gao, Ronglin He, Zhiyou Zong, Dongyuan Zhang A β-glucosidase Hyperproducing Strain, Pencillium piceum: Novel Characterization of Lignocellulolytic Enzyme Systems and Its Application in Biomass Bioconversion: Microbial Production and Application. 2018, Fungal Cellulolytic Enzymes, DOI: 10.1007/978-981-13-0749-2_5.（学术专著）

1. 宗志友，马立娟，李晨，张东远，陈树林，一种提高纤维素酶对木质纤维素的酶解效率的方法，中国发明专利，ZL201310313584.3（授权）
2. 宗志友，马立娟，赫荣琳，李晨，贾文娣，陈树林，一种从微生物发酵液中提取纤维素酶的方法，中国发明专利，ZL201210412975.6（授权）
3. 宗志友，张东远，高乐，王国坤，李晨，崔超，陈树林，一种β-葡萄糖苷酶和β-葡萄糖苷酶突变体及应用，中国发明专利，ZL201410602338.4（授权）
4. 蔡文生，宗志友，邵学广，纤维二糖水解酶突变体及其生产方法和应用，中国发明专利，ZL201811340287.7（授权）
5. 蔡文生，宗志友，邵学广，纤维二糖水解酶突变体及其应用，中国发明专利，ZL201711049170.9（授权）
6. 张东远，宗志友，高乐，王国坤，李晨，崔超，陈树林一种β-葡萄糖苷酶和β-葡萄糖苷酶突变体及应用，中国发明专利，ZL201410603781.3（授权）

技术咨询