生物医药

微生物天然药物的发现与高效合成、生物合成与耐药机制解析以及“非天然”天然药物的合成创制

Koteva, K.#, Min, X.#, Wenliang, W., Fiebig, A., Cook, M., Coombes, B.K. & Wright, G.D. Synthetic Biology Facilitates Semi-Synthetic Development of Type V Glycopeptide Antibiotics Targeting Vancomycin Resistant Enterococcus. J. Med. Chem. 2023; 66(13):9006-9022.
Anwei, H.*, Bei, L., Zixin, D., Min, X.* & Dickschat, J.S.* Cladosporin, A Highly Potent Antimalaria Drug? ChembioChem. 2023; 24(12):e202300154.
Min, X., Wenliang, W., Waglechner, N., Culp, E.J., Guitor, A.K. & Wright, G.D. Phylogeny-Informed Synthetic Biology Reveals Unprecedented Structural Novelty in Type V Glycopeptide Antibiotics. ACS Cent. Sci. 2022; 8(5), 615-626.
Alder A., Struck N.S., Min, X., Johnson J.W., Wenliang, W., Pallant, D., Cook, M.A., Rambow, J., Lemcke, S., Gilberger, T.W. & Wright, G.D. A non-reactive natural product precursor of the duocarmycin family has potent and selective antimalarial activity. Cell Chem. Biol. 2022; 29(5):840-853.
Min, X., Wenliang, W. & Wright, G.D. Glycopeptide antibiotic discovery in the genomic era. Methods Enzymol. 2022; 665:325-346.
Min, X., Wenliang, W., Waglechner, N., Culp, E.J., Guitor, A.K. & Wright, G.D. GPAHex-A synthetic biology platform for Type IV–V glycopeptide antibiotic production and discovery. Nat. Commun. 2020; 11, 5232.
Min, X.#, Fei, Z.#, Zhuo, C., Bashiri, G., Jing, W., Jiali, H., Yemin, W., Lijun, X., Xuefei, C., Shengxiong, H., Shuangjun, L., Zixin, D. & Meifeng, T. Functional Genome Mining Reveals a Class V Lanthipeptide Containing a d‐Amino Acid Introduced by an F420H2‐Dependent Reductase. Angew. Chem. Int. Ed. 2020; 59, 18029– 18035.
Min, X. & Wright, G. D. Heterologous expression-facilitated natural products’ discovery in actinomycetes. J. Ind. Microbiol. Biotechnol. 2019; 46, 415–431.
Xuefei, C., Min, X., Jin, L., Jianguo, X., Yemin, W., Shuangjun, L., Zixin, D. & Meifeng, T. Biosynthesis of Tropolones in Streptomyces spp.: Interweaving Biosynthesis and Degradation of Phenylacetic Acid and Hydroxylations on the Tropone Ring. Appl. Environ. Microbiol. 2018; 84(12), e00349-18.
Xiangyang, L., Dongxu, L., Min, X., Meifeng, T., Linquan, B., Zixin, D., Pfeifer, B.A. & Ming, J. Reconstitution of Kinamycin Biosynthesis within the Heterologous Host Streptomyces albus J1074. J. Nat. Prod. 2018; 81(1), 72-77.
Guixi, G., Xiangyang, L., Min, X., Yemin, W., Fei, Z., Lijun, X., Jin, L., Qingshan, L., Qianjin, K., Hongyu, O., Ying, W., Rohr, J., Zixin, D., Ming, J., Shuangjun, L. & Meifeng, T. Formation of an Angular Aromatic Polyketide from a Linear Anthrene Precursor via Oxidative Rearrangement. Cell Chem. Biol. 2017; 24(7), 881–891.
Xiaoqing, Z., Qiuxiang, C., Fen, Y., Xiaozheng, W., Lingxin, K., Bo, C., Min, X., Shuangjun, L., Zixin, D., Chooi, Y.H. & Delin, Y. Biosynthesis of the pyrrolidine protein synthesis inhibitor anisomycin involves novel gene ensemble and cryptic biosynthetic steps. PNAS. 2017; 114(16), 4135–4140.
Zhilong, Z., Ting, S., Min, X., Brock, N.L., Yilei, Z., Yemin, W., Zixin, D., Xiuhua, P. & Meifeng, T. Hybrubins: bipyrrole tetramic acids obtained by crosstalk between a truncated undecylprodigiosin pathway and heterologous tetramic acid biosynthetic genes. Org. Lett. 2016; 18, 572-575.
Min, X., Yemin, W., Zhilong, Z., Guixi, G., Shengxiong, H., Qianjin, K., Xinyi, H., Shuangjun, L., Xiuhua, P., Zixin, D. & Meifeng T. Functional Genome Mining for Metabolites Encoded by Large Gene Clusters through Heterologous Expression of a Whole Genome Bacterial Artificial Chromosome Library in Streptomyces spp. Appl. Environ. Microbiol. 2016; 82(19), 5795-5805.
Li, C., Yemin, W., Hang, G., Min, X., Zixin, D. & Meifeng T. High-Throughput Screening for Streptomyces Antibiotic Biosynthesis Activators. Appl. Environ. Microbiol. 2012; 78(12), 4526–4528.