生物医药

（1）微生物替代蛋白低碳制造技术研究：以丝状真菌、酵母等为底盘细胞，开发高效的基因编辑技术，重构蛋白合成代谢途径，适配高效的蛋白制造底盘细胞工厂，开发高效的微生物蛋白发酵、后处理及其食品/材料化技术体系，实施微生物蛋白产业化；
（2）微生物油脂低碳制造技术研究：以裂殖壶菌等为底盘细胞，开发高效的基因编辑和挖掘高效的油脂合成元件，重构油脂合成代谢途径，开发高产多不饱和脂肪酸、脂溶性营养化学品和医学材料等的细胞工厂，应用于食品、饲料和医学工程等行业；
（3）合成气、甲醇和甲烷等一碳化合物生物转化研究：以甲基菌、梭菌等细菌为底盘，开发高效的基因编辑技术，挖掘/设计高效固碳功能元件，构建一碳到、二碳或多碳生物转化的细胞工厂或多细胞体系，实现一碳到蛋白、油脂等高效合成。

[1] Y. Zhou, Y. Duan, L. Chen, Y. Yang, L. Ma, W. Chen, Z. Liao, J. Cai, D. Li*, Engineering dispersed mycelium morphology in Aspergillus niger for enhanced mycoprotein production via CRISPR/Cas9-mediated genome editing, Bioresource Technology 432 (2025) 132652. https://doi.org/10.1016/j.biortech.2025.132652.
[2] Y. Zhai, L. Chen, L. Ma, Y. Duan, W. Chen, L. Long, G. Wang, A. Shi, G. Chen, D. Li*, Fluorescent protein-based anaerobic reporter for construction of promoter libraries in Clostridium autoethanogenum, International Journal of Biological Macromolecules 310 (2025) 143155. https://doi.org/10.1016/j.ijbiomac.2025.143155.
[3] Y. Hu, Y. Duan, Y. Zhang, L. Ma, Y. Zhai, W. Chen, L. Long, W. Bai, Y. Shen, L. Chen, X. Ren, D. Li*, Reprogramming Schizochytrium cell factory for High-Yield production of astaxanthin via mutagenesis and metabolic engineering, Chemical Engineering Journal 515 (2025) 163556. https://doi.org/10.1016/j.cej.2025.163556.
[4] Y. Duan, L. Chen, L. Ma, Y. Zhai, Y. Hu, G. Li, G. Chen, D. Li*, CRISPR/Cas9-mediated metabolic engineering for enhanced PUFA production in Schizochytrium limacinum, Chemical Engineering Journal 517 (2025) 164320. https://doi.org/10.1016/j.cej.2025.164320.
[5] S. Tong, R. Hong, W. Chen, M. Chai, Y. Zhang, Y. Sun, Q. Wang, D. Li*, Synchronous Bioproduction of Betanin and Mycoprotein in the Engineered Edible Fungus Fusarium venenatum, Journal of Agricultural and Food Chemistry 72 (2024) 19462-19469. 10.1021/acs.jafc.4c06071.
[6] S. Tong, W. Chen, R. Hong, M. Chai, Y. Sun, Q. Wang, D. Li*, Efficient Mycoprotein Production with Low CO2 Emissions through Metabolic Engineering and Fermentation Optimization of Fusarium venenatum, Journal of Agricultural and Food Chemistry 72 (2024) 604–612. 10.1021/acs.jafc.3c08509.
[7] R. Hong, S. Tong, M. Chai, W. Chen, X. Liu, Y. Chen, D. Li*, Enhancing Mycoprotein Yield: Metabolic Modulation of Chitin Synthase in Fusarium venenatum, Journal of Agricultural and Food Chemistry 72 (2024) 27274–27283. 10.1021/acs.jafc.4c07105.
[8] Y. Duan, L. Chen, L. Ma, F.R. Amin, Y. Zhai, G. Chen, D. Li*, From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects, Biotechnology Advances 77 (2024) 108460. https://doi.org/10.1016/j.biotechadv.2024.108460.
[9] H. Cui, W. Liu, C. Ma, P. Shiri, Z. Zhu, H. Jiang, D. Li*, L. Zhang, Converting CO2 to single-cell protein via an integrated electrocatalytic-biosynthetic system, Applied Catalysis B: Environment and Energy 350 (2024) 123946. https://doi.org/10.1016/j.apcatb.2024.123946.
[10] J. Wang, L. Chen, J. Xu, S. Ma, X. Liang, Z. Wei, D. Li*, M. Xue, C1 gas protein: A potential protein substitute for advancing aquaculture sustainability, Reviews in Aquaculture 15 (2023) 1-19. https://doi.org/10.1111/raq.12707.

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