生物医药

开发和优化自动化合成生物基因编辑和调控工具以及高通量筛选技术，以期建立枯草芽孢杆菌工程菌种自动化铸造平台，提升菌种设计创制

Wang, Y. #*, Cheng, H. #, Liu, Y. #, Liu, Y., Wen, X., Zhang, K., Ni, X., Gao, N., Fan, L., Zhang, Z., Liu, J., Chen, J., Wang, L., Guo, Y., Zheng, P.*, Wang, M.*, Sun, J., & Ma, Y. (2021). In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing. Nature communications, 12(1), 678.
Liu, Y., Cheng, H., Li, H., Zhang, Y., &Wang, M.* (2023). A Programmable CRISPR/Cas9 Toolkit Improves Lycopene Production in Bacillus subtilis. Applied and environmental microbiology, 89(6), e0023023.
Liu, Y., Liu, Y., & Wang, M.* (2017). Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering. Frontiers in microbiology, 8, 2012.
Liu, Y. #, Liu, Y. #, Zheng, P., Wang, Y., & Wang, M.* (2023). Cytosine Base Editing in Bacteria. Methods in molecular biology, 2606, 219-231.
Xiao, F., Dong, S., Liu, Y., Feng, Y., Li, H., Yun, C. H., Cui, Q., & Li, W. * (2020). Structural Basis of Specificity for Carboxyl-Terminated Acyl Donors in a Bacterial Acyltransferase. Journal of the American Chemical Society, 142(37), 16031-16038.
Liu, Y., Qin, W., Liu, Q., Zhang, J., Li, H., Xu, S., Ren, P., Tian, L., & Li, W. (2016). Genome-wide identification and characterization of macrolide glycosyltransferases from a marine-derived Bacillus strain and their phylogenetic distribution. Environmental microbiology, 18(12), 4770-4781.
Qin, W.#, Liu, Y.#, Ren, P., Zhang, J., Li, H., Tian, L., & Li, W. (2014). Uncovering a glycosyltransferase provides insights into the glycosylation step during macrolactin and bacillaene biosynthesis. Chembiochem, 15(18), 2747-2753.
Liu, Y., Zheng, H., Zhan, G., Qin, W., Tian, L., & Li, W. (2014). Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain. Science China. Life sciences, 57(6), 627–635.
Yu, S. #, Price, M. A. #, Wang, Y., Liu, Y., Guo, Y., Ni, X., Rosser, S. J., Bi, C., & Wang, M. (2020). CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis. ACS synthetic biology, 9(7), 1781–1789.
Liu, Q. #, Ren, P. #, Liu, Y., Qin, W., Li, H., & Li, W. (2018). Exploration of the Glycosyltransferase BmmGT1 from a Marine-Derived Bacillus Strain as a Potential Enzyme Tool for Compound Glycol-Diversification. Journal of microbiology and biotechnology, 28(6), 931–937.