精细化工,生物医药

1．酶与酶工程：原料高值化再利用、绿色生物催化基因工程菌/酶的开发与利用，基于定向进化的酶性质及功能改造，如咖啡因代谢相关酶、辅因子再生系统、环氧水解酶等。
2．靶向性生物药物：1）基于组织特异性启动子的靶向给药、靶向RNAi体系的建立与效果评价；2）适用于癌症、皮肤病等的靶向性重组蛋白药物的研究与开发
3．不依赖于连接酶的基因重组新方法的建立与应用

1. Wu YW, Kong XD, Zhu QQ, Fan LQ, Xu JH. Chemenzymatic enantioconvergent hydrolysis of p-nitrostyrene into (R)-p-nitrophenyl glycol by a newly cloned epooxide hydrolase VrEH2 from Vigna radiate. Catalysis communications. 2015, 58(1):16-20.
2. Zhu QQ, He WH, Kong XD, Fan LQ, Zhao J, Li SX, Xu JH. Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol. Appl Microbiol Biotechnol. 2014,98(1):207-18
3. Dong HW, Fan LQ, Luo Z, Zhong JJ, Ryu DD, Bao J . Improvement of ethanol productivity and energy efficiency by degradation of inhibitors using recombinant Zymomonas mobilis (pHW20a-fdh). Biotechnol Bioeng. 2013 Sep;110(9):2395-404. doi: 10.1002/bit.24897.
4. Yang Y, Xiang K, Yang YX, Wang YW, Zhang X, Cui Y, Wang H, Zhu QQ, Fan L, Liu Y, Cao A  An individually coated near-infrared fluorescent protein as a safe and robust nanoprobe for in vivo imaging. Nanoscale. 2013 Nov 7;5(21):10345-52.
5. Shangguan JJ, Fan LQ, Ju X, Zhu QQ, Wang FJ, Zhao J, Xu JH. Expression and characterization of a novel enantioselective lipase from Aspergillus fumigatus. Appl Biochem Biotechnol. 2012,168(7):1820-33. doi: 10.1007/s12010-012-9899-x. (共同第一作者)
6. Shangguan JJ, Fan LQ, Ju X, Zhu QQ, Wang FJ, Zhao J, Xu JH. Expression and characterization of a novel enantioselective lipase from Aspergillus fumigatus. Appl Biochem Biotechnol. 2012,168 (7):1820-33.
7. Fan L, Hardy DO, Catteral JF, Zhao J, Li S. Identification and characterization of an androgen-responsive Kap promoter enhancer located in the intron II region of human angiotensinogen gene.  J Steroid Biochem Mol Biol  2010,119(3-5):135-40
8. 孟军，刘飞，范立强等. 雄激素可调节的肾脏近端肾小管上皮细胞靶向杂合启动子的优化. 华东理工大学学报， 2014:40（1）
9. 吴宝杰，范立强 响应面优化假单胞杆菌降解咖啡因培养基 科学技术与工程  2014，14（12）：17-22
10. 左丽君，吴宝杰，范立强 人乳头瘤病毒18型E6的点突变体对宫颈癌Hela细胞生长和凋亡的影响 中国生化药物杂志 2014，34（1）：51-55
11. 王刚，范立强，程安阳等。结核分枝杆菌RipB基因的克隆、表达及对藤黄微球菌生长的影响 华东理工大学学报， 2013:39（4）：
12. 李小灵，范立强 18型人乳头瘤病毒E6蛋白截短体(HPV18 E6*)的基因克隆与表达 食品与药品 2013,15（1）：6-9