生物医药

1. 微生物代谢工程技术
从微观和宏观代谢流的角度进行工业微生物细胞内功能元件的性能评测，揭示微生物细胞合成代谢调控机制，指导系统优化重构。
2. 发酵过程智能控制优化技术
建立了基于生物过程先进仪表多参数检测与采集的发酵过程可视化技术，通过多尺度生理代谢参数全息分析，构筑生物过程代谢动力学模型，实现发酵过程智能化控制。
3. 工业发酵工程设计与放大
将微生物生理代谢状态参数与反应器流程混合状态参数相结合，研究工业发酵过程多层次传质代谢动力学，实现工业反应器工艺设计和工艺精准放大。

[1] Sinan Zhang; Yuxuan Liu; Ali Mohisn; Zejian Wang*. Synergistic multi-enzyme system of Sphingobacterium sp. for enhanced penicillin G degradation: Pathways, proteomics, and binding dynamics analysis. International Journal of Biological Macromolecules,145730
[2] Sinan Zhang, Zejian Wang*, Sheng Huang, Xuheng Deng, Jiequn Wu, Shiyong Wu, Magnetic biochar derived from penicillin fermentation residue for efficient penicillin G sodium adsorption: kinetics, degradation mechanism, and simulations, Bioresource Technology, Volume 435, 2025, 132885, https://doi.org/10.1016/j.biortech.2025.132885.
[3] Sinan Zhang, Zejian Wang *, Sheng Huang, Jianmin Zhang, Jiequn Wu, Shiyong Wu, Degradation mechanism of PGNa by the immobilized degrading enzymes from magnetic fermentation residue biochar. Science of the Total Environment 963 (2025) 178432
[4] Meng Q, Xiao C, Wang Z*, Shang Y. Inhibitory Effect of Probiotic Metabolites on Seborrheic Dermatitis and Acne-Related Pathogenic Bacteria. Cosmetics. 2025; 12(1):3. https://doi.org/10.3390/cosmetics12010003
[5] Xiaoqing Song, Zishu Zhang, Junxiong Yu, Yun Zhang, Jiayun Xue, Yuanxin Guo, Jianguang Liang, Min Ren, Qingyun Ling, Ali Mohsin, Jiangchao Qian, Zejian Wang*, Yonghong Wang, Oxygen uptake rate (OUR) guided nitrogen control strategy for improving nemadectin production by Streptomyces cyaneogriseus ssp. noncyanogenus,Process Biochemistry, 2025,  ISSN 1359-5113, https://doi.org/10.1016/j.procbio.2025.01.017.
[6] Li, B., Ge, Y., Liang, J. et al. Precise regulating the specific oxygen consumption rate to strengthen the CoQ10 biosynthesis by Rhodobater sphaeroides. Bioresour. Bioprocess. 11, 106 (2024). https://doi.org/10.1186/s40643-024-00813-0
[7] Sinan Zhang; Yuxuan Liu; Ali Mohisn; Guohui Zhang; Zejian Wang* Efficient biodegradation of penicillin G sodium by Sphingobacterium sp. SQW1: Degradation mechanism and toxicity analysis Corresponding, Journal of Hazardous Materials 468 (2024) 133485.
[8] Bo Li Xinyi Chen Dujuan Zhao Zebo Liu Junming Li Muhammad Safwan Siddique Jiequn Wu Yingping Zhuang, Zejian Wang*. Physiological Metabolic Analysis of VB12 Accumulation in Ensifer adhaerens Casida A Enhanced by Oxygen Limitation. Biotechnology Journal, 2024; 19:e202400305
[9] Junxiong Yu, Yue Zhang, Hao Liu Meijin Guo, Zejian Wang,*Temporal Dynamics of Stress Response in Halomonas elongata to NaCl Shock: Physiological, Metabolomic, and Transcriptomic Insights. Microbial Cell Factories, 2024, 23(1).DOI:10.1186/s12934-024-02358-52024.
[10] Wang, Xueting, Qi Yang, Cees Haringa, Zejian Wang, Ju Chu, Yingping Zhuang and Guan Wang. “An industrial perspective on metabolic responses of Penicillium chrysogenum to periodic dissolved oxygen feast‐famine cycles in a scale‐down system.” Biotechnol Bioeng. 2024 121(10):3076-3098.
[11] Zhang, Z., Shan, P., Zhang, Z-H., He, R., Wang, Z*, Gao, X., Efficient degradation of soybean protein B3 subunit in soy sauce by ultrasound-assisted prolylendopeptidase and its primary mechanism, Food Chemistry ,2023, online.
[12] Guanxue Lai, Junxiong Yu, Zejian Wang*, Machine learning methods for predicting the key metabolic parameters of Halomonas elongata DSM 2581T. Applied microbiology and biotechnology, Applied Microbiology and Biotechnology (2023) 107:5351–5365.
[13] Zhang, Z., Shan, P., Zhang, Z-H., He, R., Xing, L., Liu, J., He, D., Ma, H., Wang, Z.,Gao, X., Efficient degradation of soybean protein B3 subunit in soy sauce by ultrasound-assisted prolylendopeptidase and its primary mechanism, Food Chemistry (2023)
[14] Zebo Liu, Linxiao Du; Nan Liu; Ali Mohsin, Xiaofeng Zhu, Huijie Sun, Bei Zhou; Zhongping Yin, Yingping Zhuang, Zejian Wang*. Insights into chlorogenic acids' efficient biosynthesis through Carthamus tinctorius cell suspension cultures and their potential mechanism as a-glucosidase inhibitors, 2023.
[15] Zebo Liu, Xiaofeng Zhu, Zejian Wang*, Meijin Guo et al. Embryogenic callus induction, cell suspension culture, and spectrum-effect relationship between antioxidant activity and polyphenols composition of Siraitia grosvenorii cultured cells. Industrial Crops & Products, 2022 (176) 114380
[16] Junxiong Yu#, Zejian Wang*#, Jing Wang, Ali Mohisn, Hao Liu, Yue Zhang, Yingping Zhuang, Meijin Guo* Physiological metabolic topology analysis of Halomonas elongata DSM 2581T in response to sodium chloride stressBiotechnology and Bioengineering2022,2: 1–17
[17] Meng Wang, Zejian Wang*, Bo Li, et.al., Accurate and online quantification of viable Rhodobacter sphaeroides cells using a flow cytometry-dielectric spectroscopy (FCM-DS) method, Talanta, 2022,245: 123448.
[18] Liu, W. , Ding, L. , Xu, J. , Shang, Y. , Wang, Z*. , & Liu, H.. Synthesis of sinapic acid modified sodium hyaluronate particles and the one-step processing of multiple pickering emulsion. Colloids and Surfaces, A. Physicochemical and Engineering Aspects, 2022, 644.
[19] Gao, X., Feng, T., Sheng, M. , Wang, ZJ. , & Ma, H.. Characterization of the aroma-active compounds in black soybean sauce, a distinctive soy sauce. Food Chemistry,2021, 298: 130334.
[20] Weilu Lin, Mingzhi Huang, Zejian Wang, Yingping Zhuang, Siliang Zhang, Modelling steady state intercellular isotopic distributions with isotopomer decomposition units Computers & Chemical Engineering, 2019,121:248-264.
[21] Weilu Lin, Zejian Wang*, Mingzhi Huang, Yingping Zhuang, Siliang Zhang, On stability analysis of cascaded linear time varying systems in dynamic isotope experiments, AIChE Journal, 2020, 66(5).
[22] Zejian Wang，Xingzi Zhang，Ping Wang，Yingping Zhuang．Oxygen Uptake Rate Controlling Strategy Balanced with Oxygen Supply for Improving Coenzyme Q10 Production by Rhodobacter sphaeroides[J]．Biotechnology and Bioprocess Engineering, 2020,25(3):459-469.
[23] Zejian Wang, Jiayun Xue, Huijie Sun, Mingxia Zhao, Yonghong Wang, Ju Chua,Yingping Zhuanga, Evaluation of mixing effect and shear stress of different impeller combinations on nemadectin fermentation. Process Biochemistry. 2020, 92: 120-129.
[24] Weilu Lin, Zejian Wang, Mingzhi Huang, Yingping Zhuang, Siliang Zhang, On structural identifiability analysis of the cascaded linear dynamic systems in isotopically non-stationary 13C labelling experiments Mathematical Biosciences, 2018,330: 122-129
[25] Zhang Qin , Sun Jingyun , Wang Zejian*, Haifeng Hanga⁎, Wei Zhao, Yingping Zhuang, Ju Chu. Kinetic analysis of curdlan production by Alcaligenes faecalis with maltose, sucrose, glucose and fructose as carbon sources.[J]. Bioresource Technology, 2018, 259:319-324
[26] Ze-Jian Wang, Hui-lin Shi, Ping Wang. The online morphology control and dynamic studies on improving vitamin B12 production by Pseudomonas denitrificans with online capacitance and specific oxygen consumption rate. Applied Biochemistry and Biotechnology, 2016, 179(6):1115 -1127

1、 2020-2024 “脱氮假单胞菌氧限制条件下NADPH对维生素B12生物合成的应激代谢调控机理研究” 32071471，项目负责人；
2、 2021-2025 基于培养环境应激响应的维生素工业菌株重构，国家重点研发计划“绿色生物制造”重点专项，子课题负责人；
3、 2013-2016 “同位素标记实验考察脱氮假单胞菌的甜菜碱代谢机制与微观代谢通量分析”，国家自然科学基金， 31200024，项目负责人
4、 2020-2024 “嗜盐菌底盘细胞代谢网络模型构建与发酵工艺优化放大研究”2020YFA096800，国家重大专项项目，子课题负责人；
5、 2017-2021 国家重点研发计划项目“微生物活菌含量计量关键技术研究”2017YFF0204602，子课题负责人；
6、 2017-2020国家科技重大专项项目“大宗抗生素发酵过程水污染物减排技术优化”2017ZX07402003，子课题负责人；
7、 2013-2016 “有机酸发酵过程关键参数在线检测与控制技术研究”， 863计划 2015AA021005， 子课题负责人；
8、 2012-2015 “基于稳定性同位素13C的细胞微观代谢通时检测系统研制及应用研究”， 国家支撑计划，2011BAF02B0，子课题负责人。
9、 2011-2014 “VB12发酵过程生产菌活细胞量检测仪研制及应用研究”，国家科技支撑计划， 2011BAF02B03，子课题负责人；
10、 2013-2014 “利福霉素高效菌株选育及发酵工艺关键技术研究”， 河北省科技支撑项目，13272803D，项目负责人；
11、 2012-2014 “生物过程关键技术及装备研发”， 863课题，2012AA021201 ，课题骨干；
12、 2012-2015 “基于宏观和微观代谢分析相结合的细胞代谢过程”，973，2012CB721006 课题骨干。