精细化工,生物医药

1、 多酶自组装与酶催化工程：胞内外的多酶自组装技术、多酶级联催化技术研究；核苷酸类产品的全酶法合成技术研究；手性药物及手性医药中间体的生物合成。对酶催化反应以及生物转化生产手性药物及手性中间体有丰富的经验。有丰富的有机合成实验室经验以及工业放大实践经验，主持完成多项新药研发工作，获得2项国家新药临床批件，熟悉新药研发及报批程序。
2、 合成生物学：天然产物的微生物合成系统构建与制备，研究复杂天然产物的模块化组装、合成途径优化、动态代谢调控策略等技术，利用微生物合成多个天然产物。
3、 核酸药物研究：主要从事反义核酸、RNAi的研究，重点研究RNA药物在肿瘤多药抗性方面的作用机制以及药理药效学研究；肿瘤药物靶向运载体系的研究；抗体药物研究等。
4、 仿生粘附材料：仿生粘附材料的制备、性能研究与应用，特别是在蛋白纯化、酶固定化、细胞培养中的应用研究。
5、 中药及其有效成分新功能开发：建立了针对前列腺增生、肥胖、皮肤瘙痒、皮肤抗皱抗衰老等天然产物体外筛选模型；中草药有效成分的分离纯化以及结构鉴定。

1．  Yuting Li, Ming Zhao, Dongzhi Wei, Jian Zhang, Yuhong Ren*. Photocontrol of itaconic acid synthesis in Escherichia coli. ACS Synthetic Biology. 2022, https://doi: 10.1021/acssynbio.2c00014.
2．  Ming Zhao, Yuting Li, Fengqing Wang, Yuhong Ren*, Dongzhi Wei. A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli. Synthetic and Systems Biotechnology. 2022, https://doi.org/10.1016/j.synbio.2022.05.008
3．  Dongni Ji, Jianhua Li, Yuhong Ren*, Yong Wang. Rational engineering in Escherichia coli for high-titer production of baicalein based on genome-scale target identification. Biotechnology and Bioengineering. 2022, doi: 10.1002/bit.28087.
4．  Zhantao Zhu, Mengmeng Du, Bei Gao, Xinyi Tao, Ming Zhao, Yuhong Ren, Fengqing Wang, Dongzhi Wei. Metabolic compartmentalization in yeast mitochondria: burden and solution for squalene overproduction. Metabolic Engineering. 2021,68: 232-245.
5．  Dongni Ji, Jianhua Li, Fanglin Xu, Yuhong Ren*, Yong Wang. Improve the biosynthesis of baicalein and scutellarein via manufacturing self-assembly enzyme reactor in vivo. ACS Synthetic Biology. 2021, 10(5):1087-1094..
6. Jianlin Xu, Haili Liu, Zhifeng Liu, Yuhong Ren, Yong Wang. Acylated aminooligosaccharides from the yellow sea Streptomyces sp. HO1518 as both α-glucosidase and lipase inhibitors. Marin Drugs. 2020, 18(11): 576-589.
7. Guosong Liu, Tian Li, Wei Zhou, Min Jiang, Xinyi Tao, Min Liu, Ming Zhao, Yuhong Ren, Bei Gao, Fengqing Wang, Dongzhi Wei. The yeast peroxisome: a dynamic storage depot and subcellular factory for squalene overproduction. Metabolic Engineering. 2020, 57: 151-161.
8. Zhaoyu Chang, Jian Zhang, Wanyuan Dong, Xiangqi Meng, Hualei Wang, Dongzhi Wei, Yuhong Ren*. Cadmium sulfide net framework nanoparticles for photo-catalyzed cell redox. RSC Advances. 2020, 10(62): 37820-37825.
9. Wanju Sun, Li Wang, Haohao Liu, Yongjun Liu, Yuhong Ren, Fengqing Wang, Dongzhi Wei. Characterization and engineering control of the effects of reactive oxygen species on the conversion of sterols to steroid synthons in Mycobacterium neoaurum. Metabolic Engineering. 2019, 56: 97-110.
10.   Tongjing Sun, Baoqi Zhang , Jinping Lin, Yuhong Ren*. Reversible Photocontrol of Oxidase Activity by Inserting a Photosensitive Domain into the Oxidase. Bioresources and Bioprocessing. 2019. 6:28-33
11.   Zhongwei Yang, Hongling Wang, Yuxiao Wang, Yuhong Ren*, Dongzhi Wei*. Manufacturing Multienzymatic Reactors In Vivo by Self-Assembly to Improve the Biosynthesis of Itaconic Acid in Escherichia coli. ACS Synthetic Biology. 2018. 7(5), 1244-1250.
12.   Pengyan Jiao, Jian Zhang, Yuguo Dong, Dongzhi Wei, Yuhong Ren*.            Construction and characterization of the recombinant immunotoxin RTA-4D5-KDEL targeting HER2/neu-positive cancer cells and locating the endoplasmic reticulum. Applied Microbiology and Biotechnology. 2018. 102:  9585–9594.
13.    Ying Liu, Xin Gao, Dongzhi Wei, Yuhong Ren*. Reversible Photocontrol  of  Lipase Activity by Incorporating a Photoswitch into the Lid Domain. Chemphotochem. 2017, 1, 393-396. DOI: 10.1002/cptc.201700045.
14.   Xingxing Zou, Jinping Lin, Xinlei Mao, Shengyun Zhao and Yuhong Ren *. Biosynthesis of L-Erythrose by Assembly of Two Key Enzymes in Gluconobacter oxydans. Journal of Agricultural and Food Chemistry. 2017, 65, 7721-7725.
15.   Zhongwei Yang, Xin Gao, Hui Xie, Fengqing Wang, Yuhong Ren* and Dongzhi Wei. Enhanced Itaconic Acid Production by Self-Assembly of Two Biosynthetic Enzymes in Escherichia coli. Biotechnology and Bioengineering. 2017, 114(2),457-462.
16.   Xinyi Tao, Ning Jia, Nenghui Cheng, Yuhong Ren, Xuni Cao, Min Liu, Dongzhi Wei*, Feng-Qing Wang*. Design and evaluation of a phospholipase D based drug delivery strategy of novel phosphatidyl-prodrug. Biomaterials. 2017. 131, 1-14
17.   Xin Gao, Chengcheng Zhao, Ting Yu, Shengli Yang, Yuhong Ren*, Dongzhi Wei. Construction of a Reusable Multi-enzyme Supramolecular Device via Disulfide Bond Locking. Chemical Communications. 2015, 51, 10131-10133.
18.   Xin Gao, Shuai Yang, Chengcheng Zhao, Yuhong Ren*, Dongzhi Wei. Artificial Multienzyme Supramolecular Device: Highly Ordered Self-assembly of Oligomeric Enzymes In Vitro and In Vivo. Angewandte Chemie. 2014, 53, 14027-14030.