论文著作：
1. Rui Li, Minglu Xu, Meng Xu, Yiquan Zhou, Tao Wen, Qinghai Liu, Dong Yi, Hualei Wang*, Dongzhi Wei.Semi-rational engineering of a distal loop region to enhance the catalytic activity and stability of leucine dehydrogenase. Journal of Agricultural and Food Chemistry, 2025, 73(5): 2961-2969.
2. Weijie Gao, Zijian Jing, Yifang Meng, Qinghai Liu, Hualei Wang*, Dongzhi Wei. Inside-out rational design of ornithine cyclodeaminase RlOCD from Rhizobium leguminosarum by a multi-region synergy strategy for efficient synthesis of L-pipecolic acid. Journal of Agricultural and Food Chemistry, 2024，72(46):25782-25790.
3. He Liu, Qian Gao, Kaiyue Zhang, Meng Xu, Hualei Wang*, Dongzhi Wei. Combining binding pocket mutagenesis and substrate tunnel engineering to improve an (R)-selective transaminase for the efficient synthesis of (R)-3-aminobutanol. Biochemical and Biophysical Research Communications, 2024, 731:150383.
4. Zhicai Wang, Minglu Xu, Youyu Xie, Meng Xu, He Liu, Dongzhi Wei, and Hualei Wang*, One-pot two-stage biocatalytic cascade to produce L‑Phosphinothricin by two enantioselective complementary aminotransferases at high substrate loading via a deracemization process. Journal of Agricultural and Food Chemistry, 2024, 72(24):13846–13855.
5. He Liu, Shixi Wang, Meng Xu, Kaiyue Zhang, Qian Gao, Hualei Wang*, Dongzhi Wei. Engineering an (R)-selective transaminase for asymmetric synthesis of (R)-3-aminobutanol. Bioorganic Chemistry, 2024, 146. 107264.
6. Yangyang Sun, Zhuzhu Tang, Yanjun Li, Yongkang Song, Hualei Wang*, Dongzhi Wei, Shengli Yang. Identification and evolution of non-traditional nitrilase from Spirosoma linguale DSM 74 with high hydration activity. Bioorganic Chemistry, 2024, 143: 107055.
7. Zhi Chen, Hualei Wang*, Dongzhi Wei. Inverting the stereoselectivity of nitrilase toward racemic isobutylsuccinonitrile via modulating the key residues in the substrate binding pocket. Molecular Catalysis, 2023, 545:113654.
8. Ke Zhang, Tingze Pan, Yangyang Sun, Zhuzhu Tang, Yuhong Ren, Hualei Wang*, Dongzhi Wei. Rational identification of a catalytically promiscuous nitrilase by predicting a unique catalytic triad motif feature through an in silico strategy. Catalysis Science & Technology, 2023,13:4932-4940.
9. Yan Liu, Xuezhao Zhong, Zi Luo, Xiangqi Meng, Rui Li, Wa Zhong, Lin Yang, Hualei Wang*, Dongzhi Wei. The identification of a robust leucine dehydrogenase from a directed soil metagenome for efficient synthesis of L-2-aminobutyric acid. Biotechnology journal, 2023,18(8):e2200590.
10. Yangyang Sun, Zhuzhu tang, Tingze pan, Ke zhang, Liuzhu wang, Xingye Zhai, Yiran Jia, Tianqi Yuan, Yujia Qian, Hualei Wang*, Dongzhi Wei, Shengli Yang. Alleviating the trade-off by site-guided function switch of nitrilase to nitrile hydratase. Molecular Catalysis, 2023, 545:113233.
11. Wenjie Ye, Jingwen Xie, Yan Liu, Yilin Wang, Yuxin Zhang, XiaoYing Yang, Lin Yang, Hualei Wang*, Dongzhi Wei. Enhancing the Activity of an Alcohol Dehydrogenase by Using Aromatic Residue Scanning at Potential Plasticity Sites. Chemistry-A European Journal, 2023, 29(25):e202203530.
12. Xiangqi Meng, Yan Liu, Lin Yang, Rui Li, Hualei Wang*, Yaling Shen, Dongzhi Wei. Rational identification of a high catalytic efficiency leucine dehydrogenase and process development for efficient synthesis of L-phenylglycine. Biotechnology journal, 2023,18(5):e2200465.
13. Wenjie Ye, Yuxin Zhang, Yilin Wang, Jingwen Xie, Yan Liu, Lin Yang, Hualei Wang*, Dongzhi Wei. Engineering a Medium-chain Alcohol Dehydrogenase for Efficient Synthesis of (S)-N-Boc-3-pyrrolidinol by Adjusting the Conformational Dynamics of Loops. ChemCatChem, 2023, 15:e202201175.
14. Ke Zhang, Tingze Pan, Liuzhu Wang, Hualei Wang*, Yuhong Ren & Dongzhi Wei. Screening and characterization of a nitrilase with significant nitrile hydratase activity. Biotechnology Letters, 2022，44, 1163–1173.
15. Lin Yang, Kaiyue Zhang, Meng Xu, Youyu Xie, Xiangqi Meng, Hualei Wang,* and Dongzhi Wei*. Mechanism-Guided Computational Design of ω-Transaminase by Reprograming of High-Energy-Barrier Steps. Angewandte Chemie International Edition, 2022, 61(52):e202212555.
16. Xiangxiang Wang, Youyu Xie, Zhicai Wang, Ke Zhang, Hualei Wang*, Dongzhi Wei. Efficient Synthesis of (S)-1-Boc-3-aminopiperidine in a Continuous Flow System Using Transaminase-Immobilized Amino-Ethylenediamine-Modified Epoxide Supports.Organic Process Research & Development, 2022, 26, 5, 1351–1359，
17. Ling He, Wenjie Ye, Youyu Xie, Qinghai Liu, Hualei Wang*, and Dongzhi Wei. Efficient Biocatalytic Synthesis of (R)-2-Chloro-1-(3,4-difluorophenyl)ethanol by the Short-Chain Dehydrogenase PpKR8 from Paraburkholderia phymatum STM815. Organic Process Research & Development, 2022, 26, 2, 278–287.
18. Youyu Xie#, Jiguo Wang#, Lin Yang, Wei Wang, Qinghai Liu, Hualei Wang*, and Dongzhi Wei. The identification and application of a robust -transaminase with high tolerance of substrate and isopropylamine from a directed soil metagenome. Catalysis Science & Technology, 2022, 12, 2162-2175.
19. Lin Yang, Yiping Zhang, Yan Liu, Dongzhi Wei, Hualei Wang*. Highly efficient synthesis of pharmaceutically relevant chiral 3-N-substituted-azacyclic alcohols using two enantiocomplementary short chain dehydrogenases. Biochemical Engineering Journal, 2021, 178, 1-9.
20. Liuzhu Wang, Yangyang Sun, Shiqing Diao, Shuiqin Jiang, Hualei Wang*, Dongzhi Wei. Rational hinge engineering of carboxylic acid reductase from Mycobacterium smegmatis enhances its catalytic efficiency. Biotechnology Journal,2022, 17(2):e2100441.
21. Xiangqi Meng, Lin Yang, Yan Liu, Hualei Wang, * Yaling Shen, Dongzhi Wei*. Identification and Rational Engineering of a High Substrate-Tolerant Leucine Dehydrogenase Effective for the Synthesis of L-tert-Leucine. ChemCatChem, 2021, 13(14), 3340-3349.
22. Liuzhu Wang#, Shiqing Diao#, Yangyang Sun, Shuiqin Jiang, Yan Liu, Hualei Wang* and Dongzhi Wei*. Rational engineering of Acinetobacter tandoii glutamate dehydrogenase for asymmetric synthesis of L-homoalanine through biocatalytic cascades. Catalysis Science & Technology, 2021,11, 4208-4215.
23. Youyu Xie, Feng Xu, Lin Yang, He Liu, Xiangyang Xu, Hualei Wang* and Dongzhi Wei*. Engineering the large pocket of an (S)-selective transaminase for asymmetric synthesis of (S)-1-amino-1-phenylpropane. Catalysis Science & Technology, 2021,11, 2461-2470.
24. Youyu Xie, Feng Xu, Lin Yang, He Liu, Xiangyang Xu, Hualei Wang*and Dongzhi Wei*. Engineering the large pocket of an (S)-selective transaminase for asymmetric synthesis of (S)-1-amino-1-phenylpropane. Catalysis Science & Technology, 2021,11:2461-2470.
25. Zhi Chen, Hualei Wang*, Lin Yang, Shuiqing Jiang, Dongzhi Wei*. Significantly enhancing the stereoselectivity of a regioselective nitrilase for production of (S)-3-cyano-5-methylhexanoic acid using MM/PBSA method. Chemical Communications, 2021,57:931-934.
26. Zeyu Yang, Wenjie Ye, Youyu Xie, Qinghai Liu, Rong Chen, Hualei Wang*, and Dongzhi Wei. Efficient Asymmetric Synthesis of Ethyl (S)-4-Chloro-3-hydroxybutyrate Using Alcohol Dehydrogenase SmADH31 with High Tolerance of Substrate and Product in a Monophasic Aqueous System. Organic Process Research & Development, 2020, 24, 6, 1068–1076
27. Zeyu Yang, Hengwei Fu, Wenjie Ye, Youyu Xie, Qinghai Liu, Hualei Wang*, Dongzhi Wei*. Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system. Catalysis Science & Technology, 2020, 10: 70-78.
授权专利：
1. 魏东芝，王华磊，吴承骏, 刘清海, 张舰，罗中华, 张长雷。 D-amino acid oxidase mutants and uses thereof in preparing L-glufosinate，美国发明专利，授权号：US12060584B2。
2. 王华磊，郭蒙，一种 L-苏氨酸醛缩酶突变体以及在 L-丝氨酸合成中的应用，中国发明专利，授权号：ZL 202210550936.6。
3. 王华磊，魏东芝，王留柱，一种催化活性提高的羧酸还原酶突变体及其编码基因、基因工程菌以及应用，中国发明专利，授权号：ZL202110852740.8。
4. 魏东芝，王华磊，刁诗晴。一种谷氨酸脱氢酶突变体及其编码基因、基因工程菌以及在制备L-2-氨基丁酸中的应用，中国发明专利，授权号：ZL 202110334184.5。
5. 王华磊, 魏东芝, 孟祥奇，一种亮氨酸脱氢酶突变体及其编码基因、基因工程菌以及在制备L-叔亮氨酸中的应用，中国发明专利，授权号： ZL202110455494.2。
6. 王华磊，魏东芝，吴承骏，刘清海，罗中华，张长雷。 (S)-转氨酶及其应用，中国发明专利，2021-04-29，授权号：ZL 202210392967.3。
7. 魏东芝，王华磊，吴承骏, 刘清海, 张舰，罗中华, 张长雷。D-氨基酸氧化酶突变体及其在制备 L-草铵膦中的应用,中国发明专利，授权号：ZL 202110475247.9
8. 王华磊, 魏东芝, 杨泽宇。一种活性和立体选择性提高的醇脱氢酶突变体及其重组载体、基因工程菌以及应用，中国发明专利，授权号： ZL202011578803.7。
9. 王华磊，魏东芝，吴承骏, 刘清海, 罗中华, 张长雷，利用生物多酶偶联法制备L-草铵膦的方法，中国发明专利，授权号：ZL 202011496575.9。
10. 魏东芝, 王华磊, 吴承骏, 刘清海, 罗中华, 张长雷。利用生物多酶偶联法制备L-草铵膦的方法，中国发明专利，授权号： ZL 202011491692.6。
11. 王华磊, 魏东芝, 谢有余。一种ω-转氨酶及其突变体、重组质粒、基因工程菌及其应用，中国发明专利，授权号：ZL 202011346097.3。
12. 魏东芝，徐向阳，宋在伟，王华磊。一种生物法制备苯乙酸的方法，中国发明专利，授权号：ZL 201611110169.8。
13. 王华磊，谢有余，王继国，范海洋，魏东芝。一种S型的ω‑转氨酶ATA‑W12及其基因和应用，中国发明专利，授权号：ZL 201611055835.2。
14. 王华磊，张一平，魏东芝，一种醇脱氢酶LC3及其基因和应用，中国发明专利，授权号：ZL 201610066375.7。
15. 王华磊，高文渊，魏东芝。酯酶est4、酯酶EST4、重组质粒和基因工程菌株及其应用，中国发明专利，授权号：ZL 201510342723.4。
16. 王华磊，吴锴，魏东芝。一种环氧化物水解酶及其编码基因和应用，中国发明专利，授权号：ZL 201410175055.8。
联系方式：
上海市梅陇路130号华东理工大学鲁华生物技术研究所302
邮编:200237
电话：021-64253715
E-mail address: hlwang@ecust.edu.cn
王华磊
上海市
研究领域：
精细化工,生物医药
研究方向：
1. 工业酶精准发掘：
开发基于定向宏基因组技术深度挖掘特殊环境微生物基因资源；建立基于公共数据库的普适性、底物特异性/高浓度耐受性的酶精准挖掘策略；拓展基于进化机制溯源的具新催化功能酶的理性发掘手段。
2. 工业酶人工定制：
采用QM/MM 联合MD深入揭示酶催化机制；开发基于机制引导的“限速过渡态重塑”虚拟改造技术；拓展针对底物/活性口袋特征匹配的个性化定制方案略；建立基于机器学习（AI）的酶结构-功能智能预测与理性设计手段。
3. 工业酶应用技术开发：
开发多酶级联协同增效技术；开发空间有序多酶定量自组装技术；开发多酶/辅酶共固定化技术；开发生物催化低成本、规模化及清洁化生产技术，实现医药、农药、化工、食品、材料等高值产品的工艺开发并其产业化落地。
所在单位：
华东理工大学
