生物医药

（1）采用多组学（单细胞转录组、代谢组、表观遗传组等）、反向遗传操作（基因编辑）等技术，鉴定介导训练免疫激活的关键细胞并解析其介导的免疫记忆机制，探索其在调控感染性器官损伤、肠炎等疾病发生发展过程中发挥的功能；
（2）以解析训练免疫激活关键分子靶标为导向，开展抗感染药物的筛选、免疫生物制品（新型疫苗、佐剂、免疫增强剂等）的设计和活性评价。

[1] Zhuang Wang, Yuanyuan Liu, Jing Hu, Xinwei You, Jin Yang, Yuanxing Zhang, Qin Liu, Dahai Yang*. Tissue-resident trained immunity in hepatocytes protects against septic liver injury in zebrafish, Cell Reports 2024,43(6): 114324.
[2] Xinwei You#, Jin Yang#, Zhuang Wang, Qiyao Wang, Qin Liu, Yuanxing Zhang, Geert F. Wiegertjes*, Dahai Yang*. Progress and perspective of trained immunity in teleost fish, Reviews in Aquaculture 2024, 16(2): 732-740.
[3] Wei Wang, Ying Wang, Jin Yang, Qin Liu, Yuanxing Zhang, Dahai Yang*. NITR12+ NK cells release perforin to mediate IgMhi B cells killing in turbot (Scophthalmus maximus), Journal of Immunology 2023, 211(11): 1693-1700.
[4]Weijie Chen#, Jianchang Huang#, Wei Wang#, Ying Wang#,Hao Chen, Qiyao Wang, Yuanxing Zhang, Qin Liu*, Dahai Yang*, Multi-tissue scRNA-seq reveals immune cell landscape of turbot (Scophthalmus maximus),Fundamental Research 2022, 2(4): 550-561.
[5]Di Mu#, Jin Yang#, Yu Jiang#, Zhuang Wang, Weijie Chen, Jianchang Huang, Yuanxing Zhang, Qin Liu, Dahai Yang*, Single-cell transcriptomic analysis reveals neutrophil as orchestrator during β-glucan-induced trained immunity in a teleost fish, Journal of Immunology 2022, 209(4): 783-795.
[6]Jiatiao Jiang, Wenwen Wang, Fei Sun, Yuanxing Zhang, Qin Liu, Dahai Yang*, Bacterial infection reinforces host metabolic flux from arginine to spermine for NLRP3 inflammasome evasion, Cell Reports 2021, 34(10): 108832.
[7] Weijie Chen, Jingjing Zhao, Di Mu, Zhuang Wang, Qin Liu, Yuanxing Zhang, Dahai Yang*, Pyroptosis mediates neutrophil extracellular trap formation during bacterial infection in zebrafish, Journal of Immunology 2021, 206(8): 1913-1922.
[8] Zhuang Wang, Zhaoyan Gu, Qing Hou, Weijie Chen, Di Mu, Yuanxing Zhang, Qin Liu, Zhihong Liu, Dahai Yang*, Zebrafish GSDMEb cleavage-gated pyroptosis drives septic acute kidney injury in vivo, Journal of Immunology 2020, 204(7): 1929-1942.
[9] Dahai Yang, Xin Zheng, Shouwen Chen, Zhuang Wang, Wenting Xu, Jinchao Tan, Mingyu Hou, Wenhui Wang, Zhaoyan Gu, Qiyao Wang, Ruilin Zhang, Yuanxing Zhang, Qin Liu*, Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish, Nature Communications 2018, 9(1): 3052.
[10] Dahai Yang, Yuan He, Raul Muñoz-Planillo, Qin Liu*, Gabriel Núñez*, Caspase-11 requires the pannexin-1 channel and the purinergic P2X7 pore to mediate pyroptosis and endotoxic shock, Immunity 2015, 43(5): 923-932.

[1] 阳大海，王壮，何晶，刘琴，王蓬勃，王启要，张元兴，提高海水养殖鱼类抗病能力的组合制剂、其制备方法及应用，专利号：202210529727.3
[2] 阳大海，王壮，姜宇，刘琴，王蓬勃，王启要，张元兴，一种大菱鲆复合免疫增强剂及其制备方法和应用，专利号：202210283589.5
[3] 阳大海，王壮，杨秋茜，刘琴，王蓬勃，张元兴，检测大菱鲆血栓细胞的试剂及其应用，专利号：202410955405 .4
[4] 阳大海，王壮，犹昕，刘光磊，刘琴，张元兴，产黑素短梗霉来源的胞外β-葡聚糖在制备训练免疫诱导剂的应用, 专利号：202510222531.3
[5] 吴宵，马悦，王启要，刘晓红，姜博尹，刘琴，阳大海，张艺蓓，一种大菱鲆圆环病毒及其应用，专利号：202410581974.7

1. 上海市教委/上海市教育发展基金会“曙光计划”，24SG31，主持，在研；
2. 国家重点研发计划青年科学家项目（海洋农业与淡水渔业科技创新专项），2022YFD2402000，主持，在研；
3. 国家重点研发计划青年科学家项目（深海和极地关键技术与装备专项)，2022YFC2804300，参加，在研；
4.国家自然科学基金优秀青年科学基金，32122090，主持，结题；
5.上海市“科技创新行动计划”面上项目，20ZR1415500，主持，结题；
6.中国科协第二批“青年人才托举工程”，2016QNRC001，主持，结题；
7.国家自然科学基金青年项目，31602187，主持，结题；
8.上海市教委“晨光计划”，16CG33，主持，结题；
9.上海市人事局“浦江人才”，2016PJD020，主持，结题。

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