生物医药

极端生理适应条件

○ 首创冬眠模式哺乳动物十三道纹地松鼠（TLGS）的诱导型多能干细胞（iPSC）；
○ 基于TLGS及人干细胞诱导分化的细胞模型，发现细胞自发冷适应的关键亚细胞机制，并成功针对这些亚细胞机制运用药物办法增强了人及大小鼠等非冬眠物种的细胞、组织和器官的体外冷存活能力；该研究结果以封面论文及当期唯一录像摘要介绍的形式发表于2018年5月的《Cell》杂志上；
○ 基于TLGS iPSC模型及人iPSC或ES模型，可继续深入研究细胞、组织和器官的自发冷适应的关键分子机制和代谢通路；
○ 基于前期研究成果，有2项美国发明专利获批，正申请多国PCT；1项美国发明专利在申；3项中国发明获批，3项在申。

1. J Yang, …, J Ou, Aging-rewired metabolic cues promote autophagy and senescence via DRAM1. Autophagy 2025; 21(12), 3142-3164;
2. H Sun, …, J Ou, X Xing, Post-Liver Transplantation Management and Specimen Collection in Daurian Ground Squirrels for Single-Cell RNA Sequencing. Journal of Visualized Experiments: Jove 2025;
3. Z Wang, …, J Ou, Z Chen, B Yang, Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels. Journal of Visualized Experiments: Jove 2025;
4. L Zhang, …, J Ou, Y Yang, Cross-species metabolomic profiling reveals phosphocholine-mediated liver protection from cold and ischemia/reperfusion. Am J Transplant. 2024; https://doi.org/10.1016/j.ajt.2024.05.018
5. X Zhang, …, J Ou, Cold-induced FOXO1 nuclear transport aids cold survival and tissue storage. Nat Comm. 2024; 15(1), 2859;
6. L Chen, …, J Ou, Synergy of 5-aminolevulinate supplement and CX3CR1 suppression promotes liver regeneration via elevated IGF-1 signalling. Cell Reports 2023; 42(8), 112984;
7. G Jin, …, J Ou, 5-aminolevulinate and CHIL3/CHI3L1 treatment amid ischemia aids liver metabolism and reduces ischemia-reperfusion injury. Theranostics 2023; 13(14), 4802;
8. Y Zhang, …, J Ou, J Yang, W Li, Y Hu, W Wu, Cold protection allows local cryotherapy in a clinical-relevant model of traumatic optic neuropathy. Elife 2022; 11, e75070;
9. X Zhang, …, J Ou, 5-Aminolevulinate improves metabolic recovery and cell survival of the liver following cold preservation. Theranostics 2022; 12 (6), 2908;
10. M Shi,…,J Ou, Y.H. Zhan, S.Y. Lin, S.C. Lin, AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1. Nat Cell Biol. 2021; 23 (3), 268;
11.
J Ou et al. Induced pluripotent stem cells as a tool for comparative physiology: lessons from the thirteen-lined ground squirrel. J Exp Biol. 2019; jeb196493 doi: 10.1242/jeb.196493;
12. J Ou et al. iPSCs from a hibernator provide a platform for studying cold adaptation and its potential medical applications. Cell, 2018; https://doi.org/10.1016/j.cell.2018.03.010
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15. J Ou et al. Synaptic pathology and therapeutic repair in adult retinoschisis mouse by AAV-RS1 transfer. J Clin Invest. 2015; 125 (7), 2891;
16. J Ou et al. Vax1/2 Genes Counteract Mitf-Induced Respecification of the Retinal Pigment Epithelium. PLoS One 2013; 8 (3), e59247;
17. K Bharti, M Gasper, J Ou et al., A Regulatory Loop Involving PAX6, MITF, and WNT Signaling Controls Retinal Pigment Epithelium Development. PLoS Genetics 2012; 8 (7), e1002757;
18. FD Carmona, J Ou et al. Development of the cornea of true moles (Talpidae): morphogenesis and expression of PAX6 and cytokeratins. J Anat. 2010; 217: 488;
19. R Kucerova, P Walczysko, B Reid, J Ou et al. The role of electrical signals in murine corneal wound re-epithelialisation. J Cell Physiol. 2011; 226: 1544;
20.
J Ou et al. Cytoskeletal and Cell Adhesion Defects in Wounded and Pax6+/– Corneal Epithelia. i 2010 ; 51: 1415;
21. FD Carmona, Glosmann M, J Ou et al. Retinal development and function in a 'blind' mole. Proc Biol Sci. 2010; 277: 1513;
22. J Ou et al. Chronic wound state exacerbated by oxidative stress in Pax6+/- aniridia-related keratopathy. J Pathol. 2008; 215: 421;
23. L Leiper, J Ou et al. Control of patterns of corneal innervation by Pax6. Invest Ophthalmol Vis Sci. 2009; 50: 2580;
24. N Dora, J Ou et al. Pax6 dosage effects on corneal development, growth and wound healing. Dev Dyn. 2008; 237: 1295;
25. L Leiper, P Walczysko, R Kucerova, J Ou et al. The roles of calcium signaling and ERK1/2 phosphorylation in a Pax6+/- mouse model of epithelial wound-healing delay. BMC Biol. 2006; 16: 27;
26. R Kucerova, J Ou et al. Cell surface glycoconjugate abnormalities and corneal epithelial wound healing in the pax6+/- mouse model of aniridia-related keratopathy. Invest Oph Vis Sci. 2006; 47: 5276;
27. J Ou et al. Stationary phase protein overproduction is a fundamental capability of Escherichia coli. Biochem Biophys Res Commu. 2004; 314: 174;
28.
L Wang, J Ou, et al. Functional expression and characterization of four novel neurotoxins from sea anemone Anthopleura sp. Biochem Biophys Res Commu. 2004; 313: 163;
29. L Peng, X Zhong, J Ou et al. High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris. J Biotech. 2004; 108: 185.