论文著作：
1.Li, W. #, Cao, P. #, Xu, P., Sun, F., Wang, C., Zhang, J., Dong, S., Wilson, J. R., Xu, D., Fan, H., Feng, Z., Zhang, X., Zhu, Q., Fan, Y., Brown, N., Justin, N., Gamblin, S. J., Li, H., Zhang, Y.*, He, J*. Rapid reconstitution of ubiquitinated nucleosome using a non-denatured histone octamer ubiquitylation approach. Cell & Bioscience, 14(1), 81. (2024)
2.Zhang, J. #, Zhao, H., Zou, B., Li, H., Dong, S., Guan, J., Wang, C., Li, W., Liu, Y., Chen, Y., Rasheed, N.*, He, J*. Cryo-EM structure and functional analysis of the chromatin remodeler RSF. Acta Crystallographica Section F: Structural Biology Communications, 80: 125-34. (2024)
3.Xue, L. #, Chang, T. #, Li, Z., Wang, C., Zhao, H., Li, M., Tang, P., Wen, X., Yu, M., Wu, J., Bao, X., Wang, X., Gong, P., He, J., Chen, X.*, Xiong, X*. Cryo-EM structures of Thogoto virus polymerase reveal unique RNA 2 transcription and replication mechanisms among orthomyxoviruses. Nature Communications 15.1 : 4620. (2024)
4.Wang, N. #, Sheng, Y. #, Liu, Y., Guo, Y., He, J., Liu, J*. Cryo-EM structures of Mycobacterium tuberculosis polynucleotide phosphorylase suggest a potential mechanism for its RNA substrate degradation. Archives of Biochemistry and Biophysics, 754, 109917. (2024)
5.Liu, B. #, Niu, X. #, Deng, Y. #, Zhang, Z. #, Wang, Y., Gao, X., Liang, H., Li, Z., Wang, Q., Cheng, Y., Chen, Q., Huang, S., Pan, Y., Su, M., Lin, X., Niu, C., Chen, Y., Yang, W., Zhang, Y., Yan, Q., He, J., Zhao, J.*, Chen, L.*, Xiong, X*. An unconventional VH1-2 antibody tolerates escape mutations and shows an antigenic hotspot on SARS-CoV-2 spike. Cell reports, 43(6), 114265. (2024)
6. Dong, S. #, Li, H. #, Wang, M. #, Rasheed, N. #, Zou, B., Gao, X., Guan, J., Li, W., Zhang, J., Wang, C., Zhou, N., Shi, X., Li, M., Zhou, M., Huang, J., Li, H., Zhang, Y., Wong, K.H., Zhang, X., Chao, W.C.H.*, and He, J*. Structural basis of nucleosome deacetylation and DNA linker tightening by Rpd3S histone deacetylase complex. Cell Research 33, 790-801. (2023)
7. Zhang, X. #, Li, Z. #, Zhang, Y. #, Liu, Y. #, Wang, J., Liu, B., Chen, Q., Wang, Q., Fu, L., Wang, P., Zhong, X., Jin, L., Yan, Q., Chen, L., He, J.*, Zhao, J. *, and Xiong, X*. Disulfide stabilization reveals conserved dynamic features between SARS-CoV-1 and SARS-CoV-2 spikes. Life Science Alliance6. (2023)
8. Yu, H. #, Liu, B. #, Zhang, Y. #, Gao, X. #, Wang, Q. #, Xiang, H. #, Peng, X. #, Xie, C. #, Wang, Y. #, Hu, P., Shi, J., Shi, Q., Zheng, P., Feng, C., Tang, G., Liu, X., Guo, L., Lin, X., Li, J., Liu, C., Huang, Y., Yang, N., Chen, Q., Li, Z., Su, M., Yan, Q., Pei, R., Chen, X., Liu, L., Hu, F., Liang, D., Ke, B., Ke, C. *, Li, F. *, He, J. *, Wang, M.*, Chen, L.*, Xiong, X.*, and Tang, X*. Somatically hypermutated antibodies isolated from SARS-CoV-2 Delta infected patients cross-neutralize heterologous variants. Nature Communications 14, 1058. (2023)
9.Zhang, X.#, Xin, J.#, Wang, Z.#, Wu, W., Liu, Y., Min, Z., Xin, Y., Liu, B., He, J., Zhang, X., and Xu, X*. Structural basis of a bi-functional malonyl-CoA reductase (MCR) from the photosynthetic green non-sulfur bacterium Roseiflexus castenholzii. Mbio14, e0323322. (2023)
10. Tang, L. #, Dong, S. #, Rasheed, N. #, Wu, H.W. #, Zhou, N. #, Li, H., Wang, M., Zheng, J.*, He, J.*, and Chao, W.C.H*. Vibrio parahaemolyticus prey targeting requires autoproteolysis-triggered dimerization of the type VI secretion system effector RhsP. Cell Reports 41, 111732. (2022)
11. He, P. #, Liu, B. #, Gao, X. #, Yan, Q. #, Pei, R. #, Sun, J., Chen, Q., Hou, R., Li, Z., Zhang, Y., Zhao, J., Sun, H., Feng, B., Wang, Q., Yi, H., Hu, P., Li, P., Zhang, Y., Chen, Z., Niu, X., Zhong, X., Jin, L., Liu, X., Qu, K., Ciazynska, K.A., Carter, A.P., Briggs, J.A.G., Chen, J., Liu, J., Chen, X.*, He, J.*, Chen, L.*, and Xiong, X*. SARS-CoV-2 Delta and Omicron variants evade population antibody response by mutations in a single spike epitope. Nature Microbiology 7, 1635-1649. (2022)
12. Chen, M. #, He, Y. #, Liu, D., Tian, L., Xu, P., Liu, X., Pan, Y., Dong, S., He, J. *, and Zhang, Y*. Structure Insights Into Photosystem I Octamer From Cyanobacteria. Frontiers in Microbiology 13, 876122. (2022)
13. Wang, X. et al. A potent human monoclonal antibody with pan-neutralizing activities directly dislocates S trimer of SARS-CoV-2 through binding both up and down forms of RBD. Signal Transduction and Targeted Therapy 7, 114. (2022)
14. Qu, K. #, Chen, Q., Ciazynska, K.A., Liu, B., Zhang, X., Wang, J., He, Y., Guan, J., He, J., Liu, T., Zhang, X., Carter, A.P., Xiong, X.*, and Briggs, J.A.G*. Engineered disulfide reveals structural dynamics of locked SARS-CoV-2 spike. PLoS Pathogens 18, e1010583. (2022)
15. Chen, M. #, Liu, X., He, Y., Li, N., He, J.*, and Zhang, Y*. Diversity Among Cyanobacterial Photosystem I Oligomers. Frontiers in Microbiology 12, 781826. (2021)
16. Zhang, C. #, Li, L., He, J., Chen, C., and Su, D*. Nonstructural protein 7 and 8 complexes of SARS-CoV-2. Protein Science 30, 873-881. (2021)
17. Zhang, C. #, Chen, Y., Li, L., Yang, Y., He, J., Chen, C. *, and Su, D*. Structural basis for the multimerization of nonstructural protein nsp9 from SARS-CoV-2. Molecular biomedicine 1, 5. (2020)
18. Frigola, J. #, He, J. #, Kinkelin, K., Pye, V.E., Renault, L., Douglas, M.E., Remus, D., Cherepanov, P. *, Costa, A. *, and Diffley, J.F.X*. Cdt1 stabilizes an open MCM ring for helicase loading. Nature communications 8, 15720. (2017)
19. He, J. #, Chao, W.C. #, Zhang, Z., Yang, J., Cronin, N., and Barford, D*. Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex. Molecular cell 50, 649-660. (2013)
20. He, J. #, Kulkarni, K., da Fonseca, P.C., Krutauz, D., Glickman, M.H., Barford, D.*, and Morris, E.P*. The structure of the 26S proteasome subunit Rpn2 reveals its PC repeat domain as a closed toroid of two concentric alpha-helical rings. Structure 20, 513-521. (2012)
21. da Fonseca, P.C. #, He, J., and Morris, E.P*. Molecular model of the human 26S proteasome. Molecular cell 46, 54-66. (2012)
何俊
广东省
研究领域：
生物医药
研究方向：
真核生物染色质的结构与功能受到众多表观调控因子在时间与空间水平上的精准调控，从而确保生命活动的有序进行。DNA组蛋白和核小体的分子结构与组成形式是造成染色质动态结构变化的基础，并与其他表观因子如组蛋白变体、组蛋白伴侣、组蛋白修饰酶和染色质重塑复合物等一起调控染色质的动态变化和功能。针对这一重要生命过程，课题组主要从以下几个方向展开研究工作：
1. 重要表观遗传调控相关复合体的功能和分子机制
染色质的表观遗传调控由相应的大分子复合物来完成，想要充分阐明表观遗传信息是如何被呈现和维持的，就必须对这些调控复合物有分子水平上的理解。课题组通过生物化学和冷冻电镜等手段，对乙酰化和泛素化相关的表观复合物开展功能和结构研究，揭示其发挥生理功能的分子构架特征。这些复合物往往与肿瘤等重要疾病发生密切相关，我们希望能进一步在分子水平上理解其参与肿瘤发生发展的致病机理，并以此鉴定出创新性药物靶标蛋白。
2.染色质动态的原位研究
常染色质和异染色质的动态变化是细胞命运转换的关键，但是这一变化的具体分子过程和调控机制还有很多未知之处。课题组致力于在细胞核内原位环境中，在传统细胞生物学研究的基础上，结合转录组、蛋白质组等组学手段和超分辨结构光成像、冷冻电子断层成像等成像手段，全面揭示多种表观因子与染色质的相互作用网络，从而在多尺度上描绘这些表观因子所主导的染色质动态变化的分子景观蓝图。
3. 基于人工智能的冷冻电镜研究
课题组致力于通过AI算法的开发与应用，一方面旨在提升Cryo-EM实验流程的自动化程度和数据收集效率；另一方面，则聚焦于对海量成像数据进行深度挖掘与分析，以期揭示更为精细、全面的生物分子结构与功能信息，从而为表观遗传调控及染色质动态学的深入研究提供强有力的技术支持与数据洞察。
主要学术成果：
何俊博士，现任中国科学院广州生物医药与健康研究院研究员、博士生导师，入选中科院高层次人才项目，并兼任中国生物物理协会冷冻电子显微学分会委员、中国电子显微镜学会广东省分会委员。主要专注于利用冷冻电镜等手段研究生物大分子的高分辨率三维结构特征，探索在生理和病理过程中起到重要作用的表观遗传调控机器的结构和分子机理。以第一作者和通讯作者身份在Cell Research、Molecular Cell、Nature Microbiology和 Nature Communication等国际高影响期刊上发表论文数十篇，多次受邀在国内外会议上作学术报告。同时承担国家重点研发计划项目、国家自然科学基金国际(地区)合作与交流项目、国家自然科学基金专项项目、国家自然科学基金面上项目、广东省自然科学基金面上项目等多个科研任务。
所在单位：
中国科学院广州生物医药与健康研究院
