生物医药

利用基因编辑工具构建大动物模型
基因修饰大动物模型在异种器官移植中的应用

1.Li N#, Gou S#, Wang J#, Zhang Q, Huang X, Xie J, Li L, Jin Q, Ouyang Z, Chen F, Ge W, Shi H, Liang Y, Zhuang Z, Zhao X, Lian M, Ye Y, Quan L, Wu H*, Lai L*, Wang K*. CRISPR/Cas9-Mediated Gene Correction in Newborn Rabbits with Hereditary Tyrosinemia Type I. Mol Ther. 2021 Mar 3;29(3):1001-1015.
2. Li W#, Shi L#, Zhuang Z#, Wu H#, Lian M, Chen Y, Li L, Ge W, Jin Q, Zhang Q, Zhao Y, Liu Z, Ouyang Z, Ye Y, Li Y, Wang H, Liao Y, Quan L, Xiao L*, Lai L*, Meng G*, Wang K*. Engineered Pigs Carrying a Gain-of-Function NLRP3 Homozygous Mutation Can Survive to Adulthood and Accurately Recapitulate Human Systemic Spontaneous Inflammatory Responses. J Immunol. 2020 Nov 1;205(9):2532-2544.
3.Lian M#, Chen F#, Huang X#, Zhao X, Gou S, Li N, Jin Q, Shi H, Liang Y, Xie J, Ge W, Zhuang Z, Wang J, Ye Y, Yang Y, Wang K*, Lai L*, Wu H*. Improving the Cpf1-mediated base editing system by combining dCas9/dead sgRNA with human APOBEC3A variants. J Genet Genomics. 2021 Jan 20;48(1):92-95.
4 .Wu H#, Liu Q#, Shi H, Xie J, Zhang Q, Ouyang Z, Li N, Yang Y, Liu Z, Zhao Y, Lai C, Ruan D, Peng J, Ge W, Chen F, Fan N, Jin Q, Liang Y, Lan T, Yang X, Wang X, Lei Z, Doevendans PA, Sluijter JPG, Wang K*, Li X*, Lai L*. Engineering CRISPR/Cpf1 with tRNA promotes genome editing capability in mammalian systems. Cell Mol Life Sci. 2018 Oct;75(19):3593-3607.
5.Yang Y#, Wu H#, Kang X#, Liang Y, Lan T, Li T, Tan T, Peng J, Zhang Q, An G, Liu Y, Yu Q, Ma Z, Lian Y, Soh BS, Chen Q, Liu P, Chen Y, Sun X, Li R, Zhen X, Liu P, Yu Y*, Li X*, Fan Y*. Targeted elimination of mutant mitochondrial DNA in MELAS-iPSCs by mitoTALENs. Protein Cell. 2018 Mar;9(3):283-297.

1.国家自然科学基金-青年项目，“利用猪全基因组sgRNA 文库筛查异种排斥靶标基因”，2020至2022年，国家级拨款/25（万元），项目负责人
2.广东省基础与应用基础研究基金项目，“猪体细胞重编程障碍分子机制研究”，2019 至 2022年，广东省拨款/10（万元），项目负责人
3.广州市科技计划项目，”通过定向进化提高Mini-CRISPR系统在哺乳动物细胞编辑效率的研究”， 2021 至 2023年，广州市拨款/5（万元），项目负责人