生物医药

基因编辑、合成生物学

Synthetic Biology;Streptomyces;Natural Products;Metabolic Engineering;Heterologous Expression;Crispr;Saccharomyces Cerevisiae;Genome Editing;Biocatalysis;Natural Product

红景天苷的微生物高效合成，链霉菌基因编辑工具开发，含氟化合物生物合成

1. Chaoqun Huang, Xuerui Jin, Zhenyu Liu, Can Cui, Yang Zhang, Binju Wang, Chun Zhang, Jianqiang Feng*, Dawen Niu*, Yunzi Luo*. Photoenzymatic enantioselective synthesis of fluorinated amides with remote stereocenter[J]. Nature Communications 2025, Jul 1;16(1):5753.
2. Qun Zhou#, Yatong Zhao#, Changqiang Ke#, Haojun Wang#, Sheng Gao, Hui Li, Yan Zhang, Yang Ye*, Yunzi Luo*. Repurposing endogenous type I-E CRISPR-Cas systems for natural product discovery in Streptomyces[J]. Nature Communications. 2024 Nov; 13;15(1):9833.
3. Jialu Li#, Huayi Liu#, Jian Li#, Juxiu Liu#, Xinli Dai, Angqi Zhu, Qingjie Xiao, Wenyu Qian, Honghao Li, Li Guo, Chuangye Yan, Dong Deng*, Yunzi Luo*, Xiang Wang*. Cryo-EM structure of the β-1,3-glucan synthase FKS1-Rho1 complex[J]. Nature Communications 2025, Feb 28;16, 2054.
4. Jialu Li#, Jian Li#, Angqi Zhu#, Xinli Dai, Juxiu Liu, Huayi Liu, Zhili Xia, Yuefan Dong, Wenyu Qian, Lunzhi Dai, Li Guo, Chuangye Yan*, Dong Deng*, Yunzi Luo*, Xiang Wang*. Structural-guided identification of two modulators of β-1,3-glucan synthase FKS1 [J]. Nature Communications 2025, Dec 7. doi: 10.1038/s41467-025-67293-4.
5. Hongbin Yu#, Zheng Wang#, Hanyue Xu, Jiusi Guo, Qingge Ma, Xiangxu Mu, Yunzi Luo*. A method for absolute protein expression quantity measurement employing insulator riboJ[J]. Engineering. 2018; 4(6): 881-887.
6. Yuhui Xie#, Xiaoyan Liu#, Tingting Wu#, Yunzi Luo*. Harnessing the Streptomyces-originating type I-E CRISPR/Cas system for efficient genome editing in Streptomyces [J]. Science China Life Science. 2025 Apr;68(4):1174-1182.
7. Chaoqun Huang#, Xuerui Jin#, Yeyi Kan, Can Cui, Longchen Wei, Huayi Liu, Dawen Niu, Yunzi Luo*. Biocatalytic synthesis of aryl and heteroaryl γ-hydroxy-α-amino Acids via an aldolase-transaminase one-pot reaction[J]. ACS Sustainable Chemistry and Engineering. 2025;13 (4): 1637-1647.
8. Xuerui Jin#, Chaoqun Huang#, Can Cui, Huayi Liu, Zhenyu Liu, Dawen Niu, and Yunzi Luo*. Chemoenzymatic synthesis of fluorinated L‑α-amino acids [J]. ACS Sustainable Chemistry and Engineering. 2024; 12 (36): 13645-13653.
9. Jian Li#, Shiqing Zhang#, Honghao Li, Xiaoran Dai, Chaoqun Huang, He Ma, Huayi Liu, Qi Qi, Xiang Sheng*, Yunzi Luo*. Engineering phenylpyruvate decarboxylase for controlled biosynthesis of aromatic amino acid derivatives[J]. Metabolic Engineering 2025, Sep; 91:466-479.
10. Huayi Liu#, Yi Zhou#, Yingjie Song#, Qianqian Zhang#, Yeyi Kan, Xinyue Tang, Qingjie Xiao, Qianyin Xiang, Huanxiang Liu*, Yunzi Luo*, and Rui Bao*. Structural and dynamics studies of the Spcas9 variant provide insights into the regulatory role of the REC1 domain[J]. ACS Catalysis. 2022; 12, 8687−8697.
11. Yunzi Luo#, Bingzhi Li#, Duo Liu#, Lu Zhang, Yan Chen, Bin Jia, Boxuan Zeng, Huimin Zhao and Yingjin Yuan*. Engineered biosynthesis of natural products in heterologous hosts[J]. Chemical Society Reviews. 2015; 44(15): 5265-5290.
12. Yunzi Luo, Hua Huang, Jing Liang, Meng Wang, Lu Lu, Zengyi Shao, Ryan E. Cobb and Huimin Zhao*. Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster[J]. Nature Communications. 2013; 4, 2894.

1. 一种FnCpf1介导的体外DNA编辑试剂盒
2. 一种高产红景天苷和/或酪醇的工程菌及其应用
3. 一种高产羟基酪醇的酿酒酵母及其构建方法
4. P450 BM3蛋白突变体及其应用
5. 一种化学-酶法制备含氟非天然氨基酸的方法

1. 酶促碳氢键氟化反应设计与构建，国家重点研发计划“合成生物学”专项
2. 基于Ⅰ类CRISPR的链霉菌转录激活系统创制与新分子发现，国家自然科学基金委项目
3. 苯丙酮酸脱羧酶的底物特异性改造及催化机制研究，国家自然科学基金委项目
4. 新型基因编辑工具库的构建及其在放线菌天然产物探索中的应用，国家自然科学基金委项目
5. 非天然多肽的定向生物智造，天津市自然科学基金青A项目
6. 活性含卤分子的生化反应设计与合成生物系统创建，京津冀基础研究合作专项
7. 新型基因编辑工具库的构建及其在放线菌天然产物探索中的应用，天津市自然科学基金项目
8. 红景天苷生物合成技术委托开发，企业合作委托项目
9. 链霉菌耐氟分子机制解析及其合成生物学应用，微生物代谢全国重点实验室开放课题

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